Journal: Neural Regeneration Research

Article Title: Electroacupuncture alleviates cerebral ischemia and reperfusion injury via modulation of the ERK1/2 signaling pathway

doi: 10.4103/1673-5374.187041

Figure Lengend Snippet: Effects of EA on mRNA expression levels of Bax, Bcl-2, GCS and Nrf2 in the ischemic cortex after MCAO. Bax (A), Bcl-2 (B), GCSh (D), GCSl (E) and Nrf2 (F) mRNA expression levels, as assessed by real-time PCR. The Bax/Bcl-2 ratio is shown in C. Data ( n = 7 per group in A–C, n = 5 per group in D–F) are expressed as the mean ± SEM. Statistical significance was determined using analysis of variance followed by Tukey’s multiple comparison test for multiple comparisons. * P < 0.05, ** P < 0.01, vs . sham (sham-operation) group; # P < 0.05, vs . MCAO group; † P < 0.05, vs. EA (MCAO + EA) group. MCAO: Middle cerebral artery occlusion; EA: electroacupuncture; EA plus PD98059: MCAO + EA + PD98059; GCSh: gamma-glutamylcysteine synthetase heavy subunit; GCSl: gamma-glutamylcysteine synthetase light subunit; Nrf2: nuclear factor erythroid 2-related factor 2.

Article Snippet: Then, the sections were incubated with rabbit anti-rat GCS monoclonal antibody (1:100; BA1627, Wuhan Boster Biotechnology Company, Wuhan, Hubei Province, China) or rabbit anti-rat Nrf2 monoclonal antibody(1:150; PB0327, Wuhan Boster Biotechnology Company), as the primary antibody, and then with horseradish peroxidase-conjugated goat anti-rabbit IgG, as the secondary antibody (1:100; BA1055, Wuhan Boster Biotechnology Company).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Comparison

Journal: Neural Regeneration Research

Article Title: Electroacupuncture alleviates cerebral ischemia and reperfusion injury via modulation of the ERK1/2 signaling pathway

doi: 10.4103/1673-5374.187041

Figure Lengend Snippet: Effects of EA on GCS and Nrf2 immunoreactivities in the ischemic cortex. (A) Immunohistochemical staining for GCS and Nrf2 in the cortex in the different groups (× 400) ( n = 5 per group). (B, C) Mean optical densities of GCS and Nrf2-immunoreactive cells. Data are expressed as the mean ± SEM. Paired t -test was used to compare two groups, while analysis of variance followed by Tukey’s multiple comparisons test was used for multiple comparisons. ** P < 0.01, vs . sham (sham-operation) group; ## P < 0.01, vs . MCAO group; †† P < 0.01, vs . EA (MCAO + EA) group. EA: Electroacupuncture; GCS: glutamylcysteine synthetase; Nrf2: nuclear factor erythroid 2-related factor 2; MCAO: middle cerebral artery occlusion; EA plus PD98059: MCAO + EA + PD98059.

Article Snippet: Then, the sections were incubated with rabbit anti-rat GCS monoclonal antibody (1:100; BA1627, Wuhan Boster Biotechnology Company, Wuhan, Hubei Province, China) or rabbit anti-rat Nrf2 monoclonal antibody(1:150; PB0327, Wuhan Boster Biotechnology Company), as the primary antibody, and then with horseradish peroxidase-conjugated goat anti-rabbit IgG, as the secondary antibody (1:100; BA1055, Wuhan Boster Biotechnology Company).

Techniques: Immunohistochemical staining, Staining

Journal: PLOS ONE

Article Title: Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway

doi: 10.1371/journal.pone.0296792

Figure Lengend Snippet: (A-E) Representative blot images and quantitative analysis of LKB1, AMPK, Nrf2, HO-1. (F-G) Representative blot images and quantitative analysis of Nuclear-Nrf2. (H-I) Representative images of Nuclear-Nrf2 and the quantitative analysis for Nuclear-Nrf2 (scale bar, 100 μm). n = 3. Data are presented as means ± S.E.M. * p < 0.05 and ** p < 0.01 vs. db/m group; Φ p < 0.05 and ΦΦ p < 0.01 vs. db/db group.

Article Snippet: Seal with 5% skim milk powder sealing solution for 2 h, adding primary antibody and incubating overnight at 4°C: Tubulin (1:10000, GTX628802) and Caspase-3(1:1000, #14220) from CST; p-AMPK (1:2000, AP0116) and HO-1(1:1000, A1346) from ABclonal; PI3K (1:1000, AF6241) from Affinity; LKB1 (1:1000, 10746-1-AP), p-LKB1 (1:5000, 80127-1-RR), AMPK (1:500, 10929-2-AP), p-AKT (1:5000, 66444-1-Ig), AKT (1:5000, 60203-2-Ig), GLUT4 (1:3000, 66846-1-Ig) from Proteintech; Bax (1:1000, GB12690) from Seville; Bcl-2 (1:1000, BA0412) and Nrf2 (1:1000, A0674) from BOSTER; Lamin-B1(1:1000, SI17-06) from Huabio.

Techniques:

Journal: PLOS ONE

Article Title: Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway

doi: 10.1371/journal.pone.0296792

Figure Lengend Snippet: (A) The cell viability of H9C2 cells was treated with HG with 0, 10, 30, and 50mM for 24 and 48 h. (B) The H9C2 cells were treated with IH and HG for 24 and 48 h, and cell viability of H9C2 cells was measured. (C) The cell viability of H9C2 cells treated with IH and HG. n = 6. (D-E) Representative images of mitochondrial membrane potential and quantitative analysis of the JC-1 ratio. n = 4. (F-G) Representative images of ROS and quantitative analysis of ROS. (H-K) Representative blot images and quantitative analysis of LKB1, AMPK, and Nrf2. n = 3. Data are presented as means ± S.E.M. * p < 0.05 and ** p < 0.01 vs. CON group; Φ p < 0.05 and ΦΦ p < 0.01 vs. HG group.

Article Snippet: Seal with 5% skim milk powder sealing solution for 2 h, adding primary antibody and incubating overnight at 4°C: Tubulin (1:10000, GTX628802) and Caspase-3(1:1000, #14220) from CST; p-AMPK (1:2000, AP0116) and HO-1(1:1000, A1346) from ABclonal; PI3K (1:1000, AF6241) from Affinity; LKB1 (1:1000, 10746-1-AP), p-LKB1 (1:5000, 80127-1-RR), AMPK (1:500, 10929-2-AP), p-AKT (1:5000, 66444-1-Ig), AKT (1:5000, 60203-2-Ig), GLUT4 (1:3000, 66846-1-Ig) from Proteintech; Bax (1:1000, GB12690) from Seville; Bcl-2 (1:1000, BA0412) and Nrf2 (1:1000, A0674) from BOSTER; Lamin-B1(1:1000, SI17-06) from Huabio.

Techniques: Membrane

Journal: PLOS ONE

Article Title: Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway

doi: 10.1371/journal.pone.0296792

Figure Lengend Snippet: (A-B) Representative images of mitochondrial membrane potential and quantitative analysis of the JC-1 ratio. (C-D) Representative images and quantitative analysis of ROS. (E-G) Representative blot images and quantitative analysis of AMPK and Nrf2. n = 3. Data are presented as means ± S.E.M. * p < 0.05 and ** p < 0.01 vs. CON group; Φ p < 0.05 and ΦΦ p < 0.01 vs. IH+HG group.

Article Snippet: Seal with 5% skim milk powder sealing solution for 2 h, adding primary antibody and incubating overnight at 4°C: Tubulin (1:10000, GTX628802) and Caspase-3(1:1000, #14220) from CST; p-AMPK (1:2000, AP0116) and HO-1(1:1000, A1346) from ABclonal; PI3K (1:1000, AF6241) from Affinity; LKB1 (1:1000, 10746-1-AP), p-LKB1 (1:5000, 80127-1-RR), AMPK (1:500, 10929-2-AP), p-AKT (1:5000, 66444-1-Ig), AKT (1:5000, 60203-2-Ig), GLUT4 (1:3000, 66846-1-Ig) from Proteintech; Bax (1:1000, GB12690) from Seville; Bcl-2 (1:1000, BA0412) and Nrf2 (1:1000, A0674) from BOSTER; Lamin-B1(1:1000, SI17-06) from Huabio.

Techniques: Membrane

Journal: International Journal of Molecular Sciences

Article Title: Impact of Extremely Low-Frequency Electromagnetic Fields on Skeletal Muscle of Sedentary Adult Mice: A Pilot Study

doi: 10.3390/ijms25189857

Figure Lengend Snippet: Anti-oxidative enzyme expression levels and activity. ( A ) Representative immunoblots of pNRF2, SOD1, SOD2, and catalase ( Left panels ) and the corresponding densitometric analyses ( Right panels ) in skeletal muscles from mice groups after 1-week exposure (0.0, 0.1, or 1.0 mT ELF-EMFs) during which they were fed a diet without or with (+NAC) NAC supplementation. ( B ) Total antioxidant status (expressed as nM Trolox equivalent/μg protein) measured by TEAC assay (see ) in the same skeletal muscle samples tested in ( A ). ( C ) Catalase enzymatic activity (expressed as µmoles H 2 O 2 /min/mL) in the same skeletal muscle samples tested in ( A ). ( D ) Representative immunoblots of pNRF2, SOD1, SOD2, and catalase ( Left panels ) and the corresponding densitometric analyses ( Right panels ) in skeletal muscle from mice groups after 5 weeks of exposure (0.0, 0.1, or 1.0 mT ELF-EMFs), during which they were fed a diet without or with (+NAC) NAC supplementation. ( E ) Total antioxidant status (expressed as nM Trolox equivalent/μg protein) measured by TEAC assay (see ) in the same skeletal muscle samples tested in ( D ). ( F ) Catalase enzymatic activity (expressed as µmoles H 2 O 2 /min/mL) in the same skeletal muscle samples tested in ( D ). All densitometric analyses were calculated as the ratio between OD × mm 2 of each band and OD × mm 2 of the corresponding loading control band (NRF2 for pNRF2, or GAPDH for SOD1, SOD2, and catalase). All data are expressed as means ± S.E.M. from six independent experiments. * p < 0.05 and ** p < 0.01 versus 0.0 mT without NAC supplementation; # p < 0.05 versus 0.0 mT with NAC supplementation.

Article Snippet: Rabbit monoclonal antibody anti-NRF2 , 1:500 , Merck Life Science S.r.l, cd SAB4501984.

Techniques: Expressing, Activity Assay, Western Blot, Muscles, Control

Journal: International Journal of Molecular Sciences

Article Title: Impact of Extremely Low-Frequency Electromagnetic Fields on Skeletal Muscle of Sedentary Adult Mice: A Pilot Study

doi: 10.3390/ijms25189857

Figure Lengend Snippet: Primary antibodies used for Western blot analyses.

Article Snippet: Rabbit monoclonal antibody anti-NRF2 , 1:500 , Merck Life Science S.r.l, cd SAB4501984.

Techniques: Western Blot, Control